Not the reaction we were expecting! Back to home. A free online platform for science education from Harvard University Since many proteins that bind DNA recognize specific sequences of bases, it is not surprising that most bind to the floor of the major groove, as this provides more chemical information for recognition than the minor groove. The major and minor grooves lie o opposite each other in the double helix, spiraling along the axis of the molecule Although several pioneer factors interact with the DNA major groove, certain nuclear receptors have dual sequence recognition, simultaneously binding the major and minor grooves A notable The use of DNA duplexes with varying hairpin constituents showed no differences in the ATHPs:DNA complex formation. The native IMS-MS, combined with the fluorescent titration and ITC data indicate that ATHP 1, 2 and 3 can bind to DNA duplexes and occupy both minor and major grooves in a and ATHP: DNA stoichiometry
DNA-Binding Motifs in Gene Regulatory Proteins
Recognize specific DNA sites through bipartite recognition of adjacent major and minor grooves by specific residues (18): the sheet interacts with the DNA major groove (GC rich sequence in available under aCC-BY-NC-ND International license By performing conformational analysis of the minor groove, it was found that mutation of the DNA central sequence A/T to C/G and deletion of the guanine 2-amino group would change the minor groove The compressed minor groove within the center of the binding site is a critical feature of the DNA structure in the Fis complex, because it enables the two recognition D α-helices of the Fis dimer to insert into adjacent major grooves. The minor groove width increases to ∼50% over its canonical width on the sides opposite to where the Fis The major groove is a wider gap that spirals up the length of the molecule, while the minor groove is a smaller gap that runs in parallel to the major groove. The The grooves twist around the molecule on opposite sides. Certain proteins bind to DNA to alter its structure or to regulate transcription (copying DNA to RNA) or replication (copying DNA to DNA). It is easier for these DNA binding proteins to interact with the bases (the internal parts of the DNA molecule) on the major groove side because the backbones To explain in words, if the glycosidic bonds (which attach the nucleic base to the sugar in the backbone) stuck straight out at 90 angles on both sides, Although several pioneer factors interact with the DNA major groove, certain nuclear receptors have dual sequence recognition, simultaneously binding
Nucleosome-bound NR5A2 structure reveals pioneer factor …
The minor groove is the opposite of the major groove in terms of its contour and size. Although it is narrower than the major groove, it is deeper and has a more refined form that provides less surface area. Triangular glyph bases fill the minor groove and, along with fewer hydrogen atoms, provide very few proteins' binding sites Analysis of the diffraction patterns of DNA has determined that there are approximately 10 bases per turn in DNA. The asymmetrical spacing of the sugar-phosphate backbones generates major grooves (where the backbone is far apart) and minor grooves (where the backbone is close together) (Figure ). These grooves are locations where proteins The presumption that water ordering in the AT-rich minor groove differs significantly from water hydrating the major groove has been a subject of Hughes, k.t., sequence-specific interaction of the salmonella hin recombinase in both major and minor grooves of dna, embo journal (). relationship between mutational changes and recombinational enhancer function or dna-binding, proceedings of the national academy of sciences of the united states of america () DNA minor groove is the target: the small molecules' attack. Go to: Abstract The macromolecule that carries genetic information, DNA, is considered Major and minor groove of DNADifferent topic of Biology includes Biotechnology, Microbiology, Immunology, Plant Physiology, and many more are available in “ Powered by Chegg AI. Share Share. The major and minor grooves in DNA play a major function in the binding of proteins and other molecu View the full answer