The sequence logo for DNA binding sites of the bacteriophage P1 replication protein RepA shows unusually high sequence conservation (∼2 bits) at a minor groove that faces RepA. However, B-form DNA can support only 1 bit of sequence conservation via contacts into the minor groove. The high conservation in RepA sites therefore implies a Major and Minor Grooves of the DNA. Types of DNA on the basis of forms. 1. A-form. 2. B-form. 3. Z-form. Types of DNA on the basis of location. 1. Nuclear DNA. So I know that the major/minor groove arise from the offset pairing of the two strands in the helix. All my textbook tells me is that "pitch causes the off-set pairing As the double helix winds up, major and minor grooves alternate on the surface of the duplex. The image on the right shows a C-G base pair, face on. Figure The major and minor grooves of the DNA double helix. From Stryer. Figure shows the major and minor grooves winding around the Double Helix. You can see that the edges of the In conclusion, both major and minor grooves of DNA play a vital role in various biological processes like replication, transcription, translation and DNA repair.
Origins of specificity in protein-DNA recognition - PMC
The B-DNA major and minor groove dimensions are crucial for DNA-protein interactions. It has long been thought that the groove dimensions depend on the Functional groups of C/G and A/T bp exposed on major and minor grooves. Proteins recognize binding sites mainly through contacts with unique functional groups in the major groove (base readout), whereas the pattern is degenerate in the minor groove. The minor-groove EP of DNA can be obtained by solving the non-linear “import numpy as npn”, “import [HOST] as pltn”, “import dnaMDn”, “%matplotlib inlinen”]}
What are the major grooves and minor grooves of DNA?
The major groove is wider than the minor groove in DNA (Figure \(\PageIndex{2d}\)), and many sequence specific proteins interact in the major groove. The twisting of the two strands around each other results in the formation of uniformly spaced major and minor grooves (Figure \(\PageIndex{4}\)). The major and minor