The major groove occurs where the backbones are far apart, the minor groove occurs where they are close together. The grooves twist around the molecule on opposite sides. Certain proteins bind to DNA to alter its structure or to regulate transcription (copying DNA to RNA) or replication (copying DNA to DNA) Achieving a partial fit of both HTH motifs into the major groove. (Right) Addition of the F1 roll parameters to the DNA in the middle panel, generating a close fit (RMSD = A
2.5: B-Form, A-Form, and Z-Form of DNA - Biology LibreTexts
Solution DNA (deoxyribonucleic acid): It is a group of molecules, which carry and mediate the genetic information from the parents to the offspring. It is formed of sugar-phosphate Each groove is lined by potential hydrogen-bond donor and acceptor atoms of the bases that enable specific interactions with proteins. The larger size of the major groove
Major and minor groove of DNA - YouTube
Figure 4: DNA has (a) a double helix structure and (b) phosphodiester bonds. The (c) major and minor grooves are binding sites for DNA binding proteins during processes such as transcription (the copying of RNA from DNA) and replication. DNA Structure is shared under a CC BY license and was authored, remixed, and/or curated The DNA-binding domain of Tn transposase uses a β-sheet to bind the major groove and a loop to engage the minor groove. However, the minor groove contacts of the Tn DNA-binding domain are predominantly with the phosphate backbone rather than with the bases, and therefore do not appear to be sequence-specific The major groove is 12 Angstroms wide, while the minor groove is 6 Angstroms wide. The major groove is slightly deeper than the minor groove ( versus Angstroms). The double helix structure of DNA contains a major groove and minor groove. In B-DNA the major groove is wider than the minor groove. [3] Given the difference in widths of High resolution three-dimensional structures for five different architectural proteins complexed to their cognate DNA sites have been solved to date and include (a) TATA An increasing number of high-resolution structures suggest that both the minor and major grooves of DNA can function as receptors for proteins and small molecules. In this review, we try to illustrate the diversity of small molecule ligands that are capable of specifically recognizing the minor groove of DNA. Complex formation results in The functional groups of minor groove stabilized DNA helix and A/B equilibrium in a cooperative manner. The equilibrium is shifted from B to A form in absence of functional groups of adenine and The energetic profiles of a significant number of protein-DNA systems at 20°C reveal that, despite comparable Gibbs free energies, association with the major groove