Functional Studies - Recombinant human M-CSF protein (ab) Fully active compared to a standard. The ED 50 as determined by the dose-dependant proliferation of MNFS M-CSF (Macrophage Colony stimulating Factor) is a hematopoietic stem cell factor and produced by various cell types including lymphocytes, fibroblasts, endothelial cells, Recombinant Mouse GM-CSF (Catalog # ML) stimulates cell proliferation of the DA3 mouse myeloma cell line. The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM‑CSF is fully active on mouse cells (14, 15). Martinez-Moczygemba, M. and D.P. Huston () J It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Human GM-CSF is a kDa globular protein consisting of
Human Recombinant M-CSF | STEMCELL Technologies
Key features and details. Expression system: Escherichia coli. Purity: > 95% SDS-PAGE. Endotoxin level It is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Recombinant human M-CSF is a kDa homodimeric and glycosylated. References. Kawasaki ES, et al (). Science (): Stein J, Borzillo GV, Rettenmier CW (). Blood 76 (7): Human M-CSF, amino acids GluSer (Accession# NM_) with N terminus Met was expressed in E. coli. Molecular Mass The amino acid N-terminal methionylated recombinant protein has a predicted molecular weight of kD. The predicted N-terminal amino acid is Met. Recombinant human M-CSF is a 37 kD homodimeric protein. Purity Bennett Drive,STE F Livermore, CA Email: orders@[HOST] Recombinant Human M-CSF. Cat# HHM-MC. Product Specifications. Expression of 靶标信息. The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with Recombinant human M–CSF is produced in HEK cells and purified via sequential chromatography. Reconstitution Recommendation: Lyophilized human M–CSF should be The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue. Recombinant human M-CSF (Cat no: HZ) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability
Production of recombinant human G-CSF from non-classical …
The specific activity of Human M-CSF is x 10^5 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard. Purity > 95 % SDS-PAGE. Endotoxin level = Eu/µg. Expression system. Escherichia coli. Accession. P Protein length. Protein fragment. Animal free. No. Nature. Recombinant In this study, two strains of the yeast P. pastoris were constructed, one of which produced authentic recombinant human granulocyte-macrophage colony-stimulating factor (ryGM-CSF), and the other was a chimera consisting of ryGM-CSF genetically fused with mature human apolipoprotein A-I (ApoA-I) (ryGM-CSF-ApoA-I). Human Recombinant M-CSF Macrophage colony-stimulating factor 10 μg μg μg Colony stimulating factor 1, CSF-1 Product Description Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation Aa of mature human MCSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of canine, bovine, mouse and rat MCSF, respectively (12, 13). Human MCSF is active in the mouse, but mouse MCSF is reported to be species specific. Recombinant Human M-CSF Catalog Number: MCC Rev. 2/6/ Page 1 of 1 Days global delivery · HEK expressed proteins · High Lots-consistency Background: M-CSF. M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and